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Monstera deliciosa
Swiss Cheese Plant

Difficulty: EasySuccess Rate: 80-85%Explant: Node

Monstera deliciosa is highly responsive to tissue culture techniques. The species demonstrates excellent regeneration capabilities from nodal explants with minimal hormone requirements.

This protocol has been optimized for node culture, which provides the most reliable results. The extended sterilization process ensures contamination-free cultures while maintaining tissue viability.

Success depends primarily on proper sterilization technique. The multi-step bleach and hydrogen peroxide treatment eliminates surface contaminants without damaging meristematic tissue.

Sterilization Protocol

  1. Step 1 Clean explants under running tap water. Use soft brush to thoroughly clean any roots or debris. Duration: until visibly clean.
  2. Step 2 Soak explants in dish soap solution with magnetic stirring. Duration: .
  3. Step 3 Prepare 1% Bleach solution (3 mL bleach to 300 mL Distilled water ) with Surfactant and Tween 20 .
  4. Step 4 Sterilize Explant s in Bleach solution without disturbing. Duration: .
  5. Step 5 Rinse with 3% hydrogen peroxide. Duration: .
  6. Step 6 Final sterilization with Hydrogen peroxide + vinegar mixture (3 mL vinegar to 150 mL H₂O₂). Duration: . Creates powerful oxidizer.

Media Formulations

Stage Components & Conditions
Stage I
Establishment 		MS medium • Sucrose 30 g/L • Agar 7 g/L • pH pH 5.7
Autoclave : 121°C, 15 PSI , 15 minutes
Stage II
Multiplication 		MS medium • Sucrose 30 g/L • Agar 7 g/L • pH pH 5.7
Monitor for media depletion after 2 weeks
Stage III
Rooting 		MS medium • Sucrose 30 g/L • Agar 7 g/L • pH pH 5.7

Culture Conditions & Transfer

Temperature: Room temperature (20°C-25°C)

Light: Standard tissue culture lighting, 16-hour Photoperiod

Subculture : Every 2 weeks if media shows signs of depletion

Humidity: 60-70% relative humidity

Acclimatization Process:

  1. Transfer rooted plantlets to small pot with sterile soil mix
  2. Gradually acclimate to ambient conditions over 2-3 weeks
  3. Reduce humidity incrementally during transition period
  4. Transfer to larger pot once well-established